Age-related decline in the expression of GDF9 and BMP15 genes in follicle fluid and granulosa cells derived from poor ovarian responders

Abstract Background Growth differentiation factor 9 ( GDF9 ) and bone morphogenetic protein 15 BMP15 genes play important roles in folliculogenesis. Altered expression of the two have been found among patients with poor ovarian response (POR). In this prospective cohort study, we determined follicle fluid (FF) granulosa cells (GCs) derived from responders grouped by age, explored its correlation outcome vitro fertilization embryo transfer (IVF-ET) treatment. Methods A total 196 POR were enrolled a tertiary teaching hospital. The diagnosed Bologna criteria sub-divided into group (< 35 year old), B (35–40 C (> 40 old). GnRH antagonist protocol was conducted for all patients, FF GCs collected after oocyte retrieval. Expression enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (qRT-PCR) Western blotting. Results Compared C, groups had significantly more pronuclei (2PN) oocytes transplantable embryos, addition higher rates implantation clinical pregnancy P < 0.05). level differed three 0.05), showing trend decline along age. ratio GDF9/BMP15 mRNA levels similar > relative proteins correlated concentrations Conclusions For responders, particular those over 40, is declined increased age accompany poorer quality IVF outcome, whilst remained relatively constant. Trial registration Chinese Clinical Registry Center ChiCTR1800016107 ). Registered on 11 May 2018.